プローブ作製関連
T7 polymerase: Roche #10881767001
T3 polymerase: Roche #11031163001
SP6 polymerase: Roche #10810274001
DIG labeling Mix: Roche #11277073910
FITC labeling Mix: Roche #11685619910
Biotin labeling Mix: Roche #11685597910
Promega: Promega Wizard® SV Gel and PCR Clean-Up System
Prinston Separation: CENTRISEP
ハイブリダイゼーション関連
Proteinase K: Roche #3115887
tRNA: Sigma #R8759-2KU
Dextran Sulfate: Sigma #D8906-50G
RNaseA: Nacalai #30141-14
Blocking Reagent: Roche #1096176
カバーガラス関連
ラック: 池本理化 803-131-11
染色バット縦型: アズワン 2-3028-01
染色バット縦型壺: アズワン 2-3029-01
PLL: SIGMA P1399
0.17mm ±0.02mm 18mm 丸型カバーガラス: Glaswarenfabrik Karl Hecht GmbH & Co. KG (特注)
一次抗体
anti-DIG mouse monoclonal[21H8]: Abcam #ab420
anti-FITC mouse monoclonal: Roche #11426320001
anti-FITC rabbit polyclonal: Abcam #ab19491
anti-FITC rabbit polyclonal: Life Technologies #A889
anti-FITC rabbit polyclonal: Life Technologies #711900
(これらのanti-FITCポリクロ3種は現ロットは同じぐらいの性能でした)
蛍光二次抗体
anti-mouse IgG Cy3 conjugated: MerckMillipore #AP124C
anti-mouse IgG Cy2 conjugated: Abcam #ab6944
anti-rabbit IgG Cy3 conjugated: MerckMillipore #AP132C
anti-rabbit IgG Cy2 conjugated: Abcam #ab6940
Streptoavidin Cy3 conjugated: Sigma #S6402
AP発色関連試薬
anti-DIG AP conjugated: Roche #1093274
anti-FITC AP conjugated: Roche #11426338910
NBT: Roche #1383213
BCIP: Roche #1383221
Levamisol: Sigma #L9756
Poly-vinyl alcohol: Sigma #363073
サンプルマウント関連
coverslips: ASSISTENT selected thickness 0.17 ± 0.01 mm 18 mm Ø #1014/18
coverslips: ASSISTENT selected thickness 0.17 ± 0.01 mm 24 mm square #1014/2424
Thiodiethanol: Sigma #166782
DABCO® 33-LV: Sigma #290734
Tetraspeck 200 nm: Life Technologies #T-7280